产品简介

YINGGUANGSU（Luciferase）SHIZIRANJIEZHONGNENGGOUCHANSHENGSHENGWUFAGUANGDEMEIDETONGCHENG，QIZHONGZUIYOUDAIBIAOXINGDESHILAIZIYINGHUOCHONGTINEI(Firefly)HEHAISHEN(Renilla)TINEIDELIANGLEIYINGGUANGSUMEI，FENBIEMINGMINGWEIF-LuciferaseHER-Luciferase。ZAIYINGGUANGSUMEIDECUIHUAXIA，YINGGUANGSU       DIWUKEYIGAOXIAODEZHUANBIANCHENGYANGYINGGUANGSU，TONGSHIFACHUQIANGLIEDEGUANG。

JIANYUCI，YANJIUZHELIYONGYINGGUANGSUMEIKAIFACHUYITAOJIQILINGMINQIESHIYONGFANGBIANDEJIYINBAOGAOXITONG。MUQIANZHEIYIXITONGBEIGUANGFANYONGYUZHUANLUYINZIJIEHEWEIDIANYUQIDONGZIHUOXINGFENXI、RNAJIANGJIE、XINHAOZHUANDAO、YAOWUSHAIXUAN、DONGWUHUOTICHENGXIANGDENGLINGYU。

两种常用载体类型极其应用场景

    pGL3-Basic---YONGYUZHUANLUYINZIJIEHEWEIDIANYUQIDONGZIHUOXINGFENXI。BADAIFENXIQIDONGZIQUDUANGOUJIANDAOF-LuciferaseSHANGYOU，HEZHUANLUYINZIGONGZHUANRANFENXIF-LuciferaseHUOXINGJIKEFANYINGQIDONGZIDEHUOXINGGAODI。GAIZHILITONGCHANGXUYAOJIEHECHIXUXINGBIAODAR-LuciferaseDEZAITIZUOWEINEICANYIXIAOZHENGBUTONGYANGPINZHIJIANZHUANRANDEZHUANRANXIAOLV(CANKAOANLIYI)。

    psiCHECK2---miRNAYUQIBADIANXIANGGUANFENXI,BAOKUOmiRNABAJIYINYANZHENG(CANKAOANLIERAHEB)、lncRNA (CANKAOANLISAN)YUcircRNA (CANKAOANLISI)XIFUmiRNAYANZHENGDENG。XUYAOBAmiRNAQIANZAIJIEHEBADIANKELONGDAOR-Luciferase (hRLuc) 3’UTRQUYU，RANHOUYUDAICEmiRNAGONGTONGZHUANRANXIBAONEICEDINGR-LuciferaseHUOXINGGAODI，F-Luciferase （hLuc+）ZUOWEIXIAOZHENGNEICANXIAOZHENGBUTONGYANGPINZHIJIANZHUANRANDEZHUANRANXIAOLV。

         技术路线

经典应用案例一

        LncRNAZHUANLUYINZIJIEHEWEIDIANYUQIDONGZIHUOXINGFENXI

 LUNWENLAIYUAN: Wang, P., et al. (2014). "The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation." Science 344(6181): 310-313.

       BENWENSHICAOXUETAOSHIYANSHIZAI2014NIANFABIAOZAIScienceSHANGYIPIANYANJIUXINGLUNWEN。ZUOZHETONGGUOXINPIANJIANDINGCHUDC (SHUTUZHUANGXIBAO)FAYUXIANGGUANDEYIGElncRNA，MINGMINGWEIlnc-DC。TONGSHIGAOTONGLIANGCEXUYEFAXIANLEZHEITIAOCHAYIlncRNADECUNZAI。JINERZUOZHETONGGUONorthern BlotYANZHENGLEZHEITIAOlnc-DC。YOUYUlnc-DCJIQITEYIHEWENDINGDIGAOBIAODAZAIDCFAYUGUOCHENGDEMOUYISHIQI，SUOYIZUOZHEJIASHElnc-DCKENENGSHIHEBIAOGUANYICHUANXUEDIAOKONGSHIJIANXIANGGUANDE。WEILEYANZHENGZHEIYIJIASHE，ZUOZHETONGGUORANSETIMIANYICHENDIANJIEHECEXUDEJISHU-CHIP-seqHEqPCRFAXIANlnc-DCDEZHUANLUQISHIWEIDIAN（TSS）FUJINFUJILEPol II、H3K4me3HEH3K27acDENGDANBAI（XIATUA），TISHIDCFAYUGUOCHENGZHONGBIAOGUANYICHUANXUEBIANHUAYUlnc-DCDEZHUANLUZENGQIANGSHIJIANXIANGDUIYING。JINERZUOZHETONGGUOXULIEFENXIFAXIANZAIlnc-DCDEZHUANLUQISHIWEIDIANFUJIN（+44-+50）CUNZAIYIGEJINGDIANDEPU.1JIEHEWEIDIAN。ZUOZHEJIASHEPU.1CANYULElnc-DCDETEYIXINGBIAODA。YUSHIZUOZHESHEJILERUXIASHIYANJINXINGLEJINYIBUDEYANZHENG（XIATUB）。

      ZUOZHEBAlnc-DCDEQIDONGZIQUDUAN（-1447-+223）JIQIBUTONGDETUBIANTIBANBENFENBIEKELONGDAOF-LuciferaseBIAODAKUANGSHANGYOU，RANHOUHEZHUANLUYINZIPU.1BIAODAZHILIHUOZHEKONGZAITI（Vector）GONGTONGZHUANRANDAOMOSHIXIBAOXIHEK-293T，TONGGUOCELIANGYINGGUANGQIANGDUDEBIANHUAJIANJIEFANYINGZHUANLUHUOXINGDEGAODI。ZUIZHONGZHENGMINGZHUANLUYINZIPU.1TONGGUOJINGDIANJIEHEWEIDIAN（+44-+50）JIEDAOLElnc-DCZAIDCFAYUGUOCHENGZHONGDETEYIBIAODA。

经典应用案例二

        LncRNATONGGUOXIFUmiRNADIAOKONGBAJIYINmRNADEBIAODA (ceRNA) 

 LUNWENLAIYUAN: Cesana, M., et al. (2011). "A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA." Cell 147(2): 358-369.

        ZUOZHETONGGUOBIAODAFENXIFAXIANLEYITIAOJIROUFAYUXIANGGUANDElncRNA---lnc-MD1.QIANQIGONGNENGYANJIUFAXIANlnc-MD1KEYIDIAOKONGJIROUFENHUADESHIJIAN。XINXIXUEFENXIFAXIANZHEITIAOJIROUTEYIDElncRNASHANGBAOHAN36GEmiRNADEJIEHEWEIDIAN。RUGUOZHIKAOLVJIROUFAYUXIANGGUANDEmiRNAHEJIYIN，36GEHOUXUANmiRNALIMIANZHISHENGXIAmiR-133HEmiR-135。ZUOZHETONGGUOYINGGUANGSUMEISHIYANJINXINGLEYANZHENG。SHOUXIANZUOZHEBAYESHENGXINGJIQIQUESHImiR-133HEmiR-135JIEHEWEIDIANDELnc-DC1KELONGDAOJINR-LucDE3’UTR，RANHOUFENBIEYUBIAODAmiR-133HEmiR-135QIANTIDEZHILIGONGTONGZHUANRANDAOXIBAOJINXINGYINGGUANGSUMEIHUOXINGJIANCE（XIATUAHEB）。ZHENGMINGlnc-MD1QUESHICUNZAImiR-133HEmiR-135DEJIEHEWEIDIAN。

      JINYIBUDEXINXIXUEYUCE，miR-133HEmiR-135FENBIEBAXIANGLIANGGEJIYIN- MAML1 HE MEF2C。TONGYANG，ZUOZHEHAISHITONGGUOYINGGUANGSUMEISHIYANJINXINGYANZHENG。YANJIUZHEBAMAML1 HE MEF2CDE3’UTR （WT）HEmiRNAJIEHEWEIDIANQUESHITUBIANTI（-mut）FENBIEKELONGZAIRLucBIAODAKUANGDEXIAYOU，RANHOUHEmiRNAGUOBIAODAZAITIGONGZHUANRANXIBAOJINXINGMEIHUOCEDING。JIEGUOXIANSHImiR-133HEmiR-135QUESHIFENBIEBAXIANGLEMAML1 HE MEF2CLIANGGEJIYIN（XIATUC）。ZUIHOUDEMOXINGSHIlnc-MD1TONGGUOceRNAJIZHIXIFUmiR-133HE135，ZHENGXIANGDIAOKONGLEMAML1 HE MEF2CDEBIAODA，CANYUDIAOKONGJIROUFAYU。

经典应用案例三

       LncRNATONGGUOXIFUmiRNADIAOKONGQISHENGLIGONGNENG (ceRNA) 

LUNWENLAIYUAN: Kallen, A. N., et al. (2013). "The imprinted H19 lncRNA antagonizes let-7 microRNAs." Molecular Cell 52(1): 101-112.

       H19SHUYUFEICHANGBAOSHOUDEYINJIJIYINJIAZU，ZAIPEITAIFAYUHESHENGZHANGFANGMIANFAHUIJIQIZHONGYAODEGONGNENG。WEILEJINYIBUYANJIUlncRNA H19DEGONGNENG，ZUOZHETONGGUOXINXIXUEFENXIFAXIANH19BAOHANlet-7DE4GEJIEHEWEIDIAN。YUSHIZUOZHETUICEH19KENENGHUIYINGXIANGlet-7FAHUIZUOYONG。

       WEILEYANZHENGZHEIYIYUCE，YANJIUZHEBA4GElet-7QIANZAIJIEHEWEIDIANCHUANLIANQILAIKELONGDAORLucDE3’UTRQU。RANHOUXIANHOUGENlet-7DEYIZHIXINGZAITI（XIATUAHEB）HEGUOBIAODAZAITI（XIATUC）FENBIEGONGZHUANRANDAOXIBAOXIJIANCEYINGGUANGSUMEIDEHUOXING，ZHENGMINGLet-7DEJIEHEWEIDIANQUESHICUNZAIQIEFAHUIGONGNENG。JINYIBUZHENGMINGH19TONGGUORNA spongeDEJIZHIYINGXIANGlet-7JIAZUBAJIYINDEBIAODACANYUJIROUFAYUDIAOKONGGUOCHENG。

经典应用案例四

       circRNATONGGUOXIFUmiRNADIAOKONGQIBAJIYINmRNADEBIAODA (ceRNA) 

LUNWENLAIYUAN: Zheng, Q., et al. (2016). "Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs." Nat Commun 7: 11215.

       HUANZHUANGRNA (circRNA)SHIFEIBIANMARNALINGYUYILEIXINDEMINGXINGFENZI，YANJIUZHEDUIQIGONGNENGHAIZHIZHISHENSHAO。

       BENWENZHONG，ZUOZHEJIANDINGCHUYIGEBIAODALIANGJIAOGAODEHUANZHUANGRNAFENZI-circHIPK3. circHIPK3LAIYUANYUJIYINHIPK3DIERGEWAIXIANZI。RNAiGANRAOcircHIPK3KEYIYINGXIANGXIBAODESHENGZHANG。WEILEJINYIBUYANJIUQIJIZHI，ZUOZHETONGGUOXULIEFENXIFAXIANcircHIPK3HANYOUYIXIELIEmiRNAJIEHEWEIDIAN。WEILECONGZHONGSHAIXUANCHUZHENZHENGDEmiRNA，ZUOZHEGOUJIANLEJIYUYINGGUANGSUMEIDESHAIXUANXITONG---BAcircHIPK3GOUJIANDAOLuciferaseDE3’UTRQUYU，RANHOUFENBIEYU424GEmiRNA mimicsGONGZHUANRANDAOMOSHIXIBAOXIHEK-293TNEIJINXINGSHAIXUANYANZHENG，ZUIZHONGZUOZHESHAIXUANCHU9GEHOUXUANDEmiRNA。TONGGUOGONGNENGSHIYANHEGONGBIAODAFENXIJINYIBUYANZHENGDEDAOmir-124SHIYANGXINGHOUXUANmiRNA。ZUIZHONGZUOZHEZHENGMINGcircHIPK3TONGGUOXIFUmiR-124ZHENGXIANGDIAOKONGLEQIBAJIYINIL6R HE DLX2DEBIAODA，YINGXIANGLEXIBAODESHENGZHANG。