自噬LC3双标腺病毒

 

自噬LC3双标腺病毒产品参数

 

 

 

自噬简介

 

XIBAOZISHI(autophagy or autophagocytosis):YOUCHENGWEIⅡXINGXIBAOSIWANG,SHIXIBAOZAIZISHIXIANGGUANJIYIN(autophagy related gene,Atg)DEDIAOKONGXIALIYONGRONGMEITIJIANGJIEZISHENSHOUSUNDEXIBAOQIHEDAFENZIWUZHIDEGUOCHENG。


比利时科学家Christian de Duve在上世纪50年代经过电镜察看到自噬体(autophagosome)构造,并且在 1963 年溶酶体国际会议上首先提出了“自噬”这种说法。因而Christian de Duve被公以为自噬研讨的鼻祖。Christian de Duve 也因发现溶酶体,于1974年取得诺贝尔奖。

 

 

MUQIANGENJUFASHENGGUOCHENGFENWEISANLEI:Macroautophagy,MicroautophagyHEChaperone-mediated autophagy CMA)。

 

● DAZISHI(Macroautophagy):JIWOMENSHUODEZISHI(autophagy);

● WEIZISHI(Microautophagy):SHIZHIRONGMEITIZHUDONG、ZHIJIETUNSHIBAOJIANGCHENGFENDEYIZHONGFANGSHI;

● FENZIBANLVJIEDAODEZISHI (Chaperone-mediated autophagy,CMA):YIXIEFENZIBANLV,RUhsp70,NENGBANGZHUWEIZHEDIEDANBAIZHUANWEIRURONGMEITI。TONGCHANGSHUODEZISHIFANZHIMacroautophagy.

 

 

自噬的过程

 

1):细胞接受自噬诱导信号后,在胞浆的某处形成一个小的类似“脂质体”样的膜结构,然后不断扩张,被称为Phagophore。
2):Phagophore不断延伸,将胞浆中的任何成分,全部揽入,然后“收口”,成为密闭的球状的autophagosome,即“自噬体”。
3):自噬体形成后,可与细胞内吞的吞噬泡、吞饮泡和内体融合(这种情况不是必然要发生的)。
4):自噬体与溶酶体融合形成autolysosome,期间自噬体的内膜被溶酶体酶降解,2者的内容物合为一体,自噬体中的“货物”也被降解,产物(氨基酸、脂肪酸等)被输送到胞浆中供细胞重新利用,而残渣或被排出细胞外或滞留在胞浆中。


 

 

自噬的调控

 

YI、YILAImTOR(BURUDONGWULEIPAMEISUBADIAN)TUJINGDEZISHI

1)PI3K-AKT-mTORXINHAOTONGLU

2)AMPK-TSC 1/2-mTOR XINHAOTONGLU

 

ER、QITADEXINHAOTONGLU

1)3-JIAJIXIANPIAOLING(3-MA)TONGGUOYIZHIClass Ⅲ PI3KDEHUOXINGYIZHIZISHI。

2)beclin1HEUVRAGZUOWEIZHENGDIAOKONGZI,KANGDIAOWANGYINZIbcl-2ZUOWEIFUDIAOKONGZIGONGTONGCANYUZUCHENGClass Ⅲ PI3 FUHEWUDIAOKONGZISHI。

3)GTPJIEHEDEGDANBAIYAJIGαi3YIZHIZISHI;GDPJIEHEDEGαi3DANBAIHUOHUAZISHI。

4)SIWANGXIANGGUANDANBAIJIMEI(death-associated protein linase,DAPK)HEDAPKXIANGGUANDANBAIJIMEI(DAPK-related protein kinase-1,DRP-1)YOUDAOZISHI。

5)PKA、caseinJIMEI Ⅱ、MAPJIMEI、calciumTUJINGYEZAIZISHICUOZONGFUZADEDIAOKONGWANGGEZHONG,DANQIJIZHIHAIBUSHENQINGCHU。

 

 

 

 

自噬与疾病

 

1、ZISHIYUDAIXIE

ZISHINENGQINGCHUBUZHENGCHANGGOUXINGDEDANBAIZHI,BINGXIAOHUASHOUSUNHEDUOYUDEXIBAOQI,SHIZHENHEXIBAOZHONGGUANGFANCUNZAIDEJIANGJIE/ZAIXUNHUANXITONG。ZAIXIBAOXINCHENDAIXIE、JIEGOUZHONGJIAN、SHENGZHANGFAYUZHONGQIZHEZHONGYAOZUOYONG。ZAIJIEHEXINSHENGERZAOQI,ZISHIZUOYONGMINGXIANJIAQIANG,ZISHITIXIANZHUZENGDUO。

 

2、ZISHIYUZHONGLIU

XIBAOZISHIYUZHONGLIUDEGUANXISHIFENFUZA,MUQIANSHANGWEIWANQUANCHANMING。YIFANGMIAN,ZHENGCHANGXIBAOZISHIZENGQIANG,KEBIAOXIANCHUYIZHIZHONGLIUFASHENGDEGONGNENG;YUCIXIANGFAN,YIZHIXIBAOZISHIYOUQIANZAIDEZHILIUKENENG。LINGYIFANGMIAN,ZHONGLIUXIBAOYEKETONGGUOZENGQIANGXIBAOZISHILAIDUIKANGYOUQUEYANG、DAIXIECHANWU、ZHILIAOYAOWUYOUDAODEYINGJIFANYING。

  

 

汉恒自噬产品与服务

 

ZISHIYIZHIJI

1)3-Methyladenine(3-MA):(Class III PI3K) hVps34 抑制剂
2)Bafilomycin A1:质子泵抑制剂
3)Hydroxychloroquine(羟氯喹)

 

ZISHIXIANGGUANKANGTI

1)A14292,PremoZISHITB/ GFP TR-FRET LC3BKANGTISHIJIHE

2)Anti-MAP1A/LC3A/B ZISHIWEIGUANXIANGGUANDANBAIQINGLIAN3KANGTI

3)Anti-MAP1LC3A(microtubule-associated protein 1 light chain 3)  ZISHIWEIGUANXIANGGUANDANBAIQINGLIAN3KANGTI

4)TUKANGREN、DA、XIAOAPG4BXIBAOZISHIXIANGGUANKANGTI\ Anti-APG4B/AUTL1

5)ZISHIWEIGUANXIANGGUANDANBAIQINGLIAN3KANGTI\ Anti-MAP1LC3A

6)Anti-SQSTM1 / p62 antibody

HANHENGSHENGWUYIKAIFABINGWANSHANLEDUOTAOZISHIXIANGGUANDEYANJIUTIXIHEGONGJU,BINGJILEILEFENGFUDEZISHIYANJIUJINGYAN

 

 

汉恒独家推出双荧光LC3细胞自噬腺病毒 

 

SHUANGYINGGUANGZHISHITIXIYUANLI

 

 

mRFP YONGYUBIAOJIJIZHUIZONGLC3,GFP DEJIANRUOKEZHISHIRONGMEITIYUZISHIXIAOTIDERONGHEXINGCHENGZISHIRONGMEITI,JIYOUYUGFP YINGGUANGDANBAIDUISUANXINGMINGAN,DANGZISHITIYURONGMEITIRONGHEHOUGFP YINGGUANGFASHENGCUIMIE,CISHIZHINENGJIANCEDAOHONGSEYINGGUANG。

 

WOMENZAIXIANWEIJINGCHENGXIANGHOUHONGLVYINGGUANGmergeHOUTONGGUOmergeHOUCHUXIANDEHUANGSEBANDIANJIZHISHIZISHITI.HONGSEDEBANDIANZHISHIZISHIRONGMEITI,TONGGUOBUTONGYANSEBANDIANDEJISHUKEYIQINGXIDEKANCHUZISHILIUDEQIANGRUO。

RUXIATU:XIBAOZHUANRANmRFP-GFP-LC3BINGDUHOUJIYUANJISUANBODUOCHULI2XIAOSHIHOUCHUXIANMINGXIANZENGQIANGDEZISHIYIJIZISHILIU。

 

HONGSEBANDIANSHIZISHIRONGMEITI(mRFP),HUANGSEBANDIANSHIZISHITI(RFP+GFP). TONGGUOBUTONGYANSEBANDIANDEJISHUKEYIQINGXIKANCHUZISHILIUDEQIANGRUO,SHISHIJIANCEZISHIFASHENGGUOCHENG,ZHUNQUE,QINGXI,ZHIGUAN!

 

 

部分客户发表文献节选:

 

● Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy

   (ZAZHI:CELL,IF=36.216,SHANGHAIJIAOTONGDAXUEFUSHURENJIYIYUAN)

● Melatonin ameliorates hypoglycemic stress-induced brain endothelial tight junction injury by inhibiting protein nitration of TP53-induced glycolysis and apoptosis regulator

   (ZAZHI:journal of pineal research,IF=15.211,ZHEJIANGDAXUE)

● The immunoproteasome catalytic ?5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation

   (ZAZHI:SCIENCE ADVANCES,IF=12.804,DALIANYIKEDAXUEFUSHUDIYIYIYUAN)

● Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling

   (ZAZHI:Nature Communications,IF=11.878,SHANDONGDAXUE)

● TRB3 links insulin/IGF to tumour promotion by interacting with p62 and impeding autophagic/proteasomal degradations

   (ZAZHI:Nature Communications,IF=11.878,BEIJINGXIEHEYIYUAN)

● Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury: Involvement of PARK2-dependent mitophagy

   (ZAZHI:Autophagy,IF=11.059,ZHEJIANGDAXUE)

● PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression

   (ZAZHI:Autophagy,IF=11.059,DIERJUNYIDAXUEFUSHUDONGFANGGANDANYIYUAN)

● Mir143-BBC3 cascade reduces microglial survival via interplay between apoptosis and autophagy: Implications for methamphetamine-mediated neurotoxicity

   (ZAZHI:Autophagy,IF=11.059,DONGNANDAXUE)

● BNIP3L/NIX-mediated mitophagy protects against ischemic brain injury independent of PARK2

   (ZAZHI:Autophagy,IF=11.059,ZHEJIANGDAXUE)

● Ai-lncRNA EGOT enhancing autophagy sensitizes paclitaxel cytotoxicity via upregulation of ITPR1 expression by RNARNA and RNA-protein interactions in human cancer

   (ZAZHI:molecular cancer,IF=10.679,HAERBINYIKEDAXUE)

● Iron oxide nanoparticles promote macrophage autophagy and inflammatory response through activation of toll-like Receptor-4 signaling

   (ZAZHI:Biomaterials,IF=10.273,SICHUANDAXUE)

● The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway

   (ZAZHI:Cell Death and Differentiation,IF=8.339,ZHONGSHANDAXUE)

● PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy

   (ZAZHI:Theranostics,IF=8.063,XIANJIAOTONGDAXUE)

 

Chinese, Simplified
 
服务分类: